Hydrogen peroxide contributes to cadmium binding on root cell wall pectin of cadmium-safe rice line (Oryza sativa L.)

Abstract

Cell wall pectin is essential for cadmium (Cd) accumulation in rice roots and hydrogen peroxide (H2O2) plays an important role as a signaling molecule in cell wall modification. The role of H2O2 in Cd binding in cell wall pectin is unclear. D62B, a Cd-safe rice line, was found to show a greater Cd binding capacity in the root cell wall than a high Cd-accumulating rice line of Wujin4B. In this study, we further investigated the mechanism of the role of H2O2 in Cd binding in root cell wall pectin of D62B compared with Wujin4B. Cd treatment significantly increased the H2O2 concentration and pectin methyl esterase (PME) activity in the roots of D62B and Wujin4B by 22.45–42.44% and 12.15–15.07%, respectively. The H2O2 concentration and PME activity significantly decreased in the roots of both rice lines when H2O2 was scavenged by 4-hydroxy-Tempo. The PME activity of D62B was higher than that of Wujin4B. The concentrations of high and low methyl-esterified pectin in the roots of D62B significantly increased when exposed to Cd alone but significantly decreased when exposed to Cd and exogenous 4-hydroxy-Tempo. No significant difference was detected in Wujin4B. Exogenous 4-hydroxy-Tempo significantly decreased the Cd concentration in the cell wall pectin in both rice lines. The modification of H2O2 in Cd binding was further explored by adding H2O2. The maximum Cd adsorption amounts on the root cell walls of both rice lines were improved by exogenous H2O2·H2O2 treatment significantly influenced the relative peak area of the main functional groups (hydroxyl, carboxyl), and the groups intensely shifted after Cd adsorption in the root cell wall of D62B, while there was no significant difference in Wujin4B. In conclusion, Cd stress stimulated the production of H2O2, thus promoting pectin biosynthesis and demethylation and releasing relative functional groups involved in Cd binding on cell wall pectin, which is beneficial for Cd retention in the roots of Cd-safe rice line.