Abstract
ZnO nanorods (ZnO–NRs) were synthesized by microemulsion-based hydrothermal method with a rapid and simple solution-based growth process without complicated equipment and the highly efficient H2O2 biosensor was fabricated on the basis of the complex films of hemoglobin (Hb), ZnO nanorods, poly (vinyl alcohol) (PVA) dispersed solution. Biocompatible ZnO–NRs–PVA composition provided a suitable microenvironment to keep Hb bioactivity (Michaelis–Menten constant of 0.0019μM), the electrochemical behaviors and effects of solution pH values were carefully examined in this paper. The Hb–ZnO–NRs/PVA biosensor demonstrated excellently electrocatalytical ability for H2O2. This biosensor had good linear relationships was obtained in the concentration range from 0.01 to 3.2μM with the detection limit of 0.009μM (S/N=3) under the optimum conditions. Moreover, the stability and reproducibility of this biosensor were evaluated with satisfactory results.
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