Abstract
BackgroundGastric cancer is one of the most prevalent and deadly malignancies without efficient treatment option. This study aimed to investigate the effect of hydrogen gas on the behavior of gastric cancer cells.MethodsGastric cancer cell lines MGC-803 and BGC-823 were treated with or without H2 /O2 gas mixture (66.7%:33.3% v/v). Proliferation and migration were assessed by MTT and scratch wound healing assays respectively. The expression of lncRNA MALAT1, miR-124-3p, and EZH2 was analyzed by real-time quantitative PCR and/or western blot. Tumor growth was estimated using xenograft mouse model.ResultsH2 gas significantly inhibited gastric tumor growth in vivo and the proliferation, migration, and lncRNA MALAT1 and EZH2 expression of gastric cancer cells while upregulated miR-124-3p expression. LncRNA MALAT1 overexpression abolished all the aforementioned effects of H2. LncRNA MALAT1 and miR-124-3p reciprocally inhibited the expression of each other. MiR-124-3p mimics abrogated lncRNA MALAT1 promoted EZH2 expression and gastric cancer cell proliferation and migration.ConclusionsThese data demonstrated that H2 might be developed as a therapeutics of gastric cancer and lncRNA MALAT1/miR-124-3p/EZH2 axis could be a target for intervention.
Highlights
Gastric cancer is one of the most prevalent and deadly malignancies without efficient treatment option
Hydrogen gas inhibited the expression of Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) and EZH2 Microarray analysis identified that H2 gas significantly inhibited the expression of Long noncoding RNA (lncRNA) MALAT1 and transcription factor EZH2 of MGC-823 cells (Fig. 2a)
LncRNA MALAT1 relieved the inhibitory effect of H2 on gastric cancer cell proliferation and migration As previously showed that H2 gas inhibited the proliferation and migration of MGC-803 and BGC-823 cells, we examined the roles of LncRNA MALAT1 on the effects of H2
Summary
Gastric cancer is one of the most prevalent and deadly malignancies without efficient treatment option. This study aimed to investigate the effect of hydrogen gas on the behavior of gastric cancer cells. LncRNA MALAT1 enhanced the stemness, proliferation, migration, invasion, and drug resistance of gastric cancer cells [17,18,19,20]. Increasing evidence showed the potential of hydrogen gas in preventing and relieving different cancers [23]. Drinking hydrogen-rich water for 6 weeks reduced reactive oxygen metabolites in the blood, maintained blood oxidation potential, improved the quality of life of malignant liver cancer patients after radiotherapy [24]. This study aims to explore the effects of hydrogen gas on gastric cancer cells and the underlying molecular mechanism
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