Abstract
Hydrogen is an eco-friendly, clean, sustainable and high energy fuel. It does not contribute to global warming and is a promising alternative to fossil fuel. Rice straw, a ligno-cellulosic material is economical, renewable and easily accessible and can be used as feed stocks for hydrogen production, through enzymatic hydrolysis. During this process sugar is released from rice straw hydrolyzate which is converted to hydrogen gas by bacteria via dark fermentation. It is a two-stage approach. In present research work, pretreatment with hydrogen peroxide, sulphuric acid and sodium hydroxide was persuaded to increase the fermentability of rice straw which was used as a substrate for hydrogen. Under dark fermentation, bacterial strains were able to procreate hydrogen gas from rice straw hydrolyzates of different pretreatments for delignification. Maximum reducing sugar was attained through H2O2 pretreatment (1453 µg/ml) compared to H2SO4 pretreatment (1376 µg/ml) and NaOH pretreatment (1334 µg/ml) under optimum conditions. Hydrogen gas is generated maximum during H2O2 pretreatment (46.99%).
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