Abstract
Gloeorercospora sorghi D. Bain & Edg., the causal agent of zonate leaf spot of Sorghum spp., is adaptively tolerant of hydrogen cyanide. Associated with adaptation to hydrogen cyanide is the appearance of formamide hydro-lyase. The enzyme is induced by hydrogen cyanide and converts hydrogen cyanide to formamide, a compound which is relatively non-toxic, Formamide hydro-lyase from G. sorghi has characteristics of a high molecular weight enzyme, is stable to storage at 4 °C, has optimal activity in the range of pH 7 to 9, and has a k m of approximately 30 m m-hydrogen cyanide. Sorghum tissue infected with G. sorghi has formamide hydro-lyase activity whereas healthy sorghum does not. Formamide hydro-lyase from infected tissue had properties similar to that produced by G. sorghi in vitro.
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