Abstract
In this study, δ 2H values of honey were detected and then applied to the identification of honey adulteration. In the meantime, δ 13C values of honey, its protein fraction, and individual sugar (glucose and fructose) were also determined by EA-IRMS and LC-IRMS, which combined with δ 2H values of honey, have been developed to improve isotopic methods devoted to the research of honey authenticity. For this purpose, 58 Chinese commercial honey samples from various origins were analyzed. The results indicated that the ranges of δ 13Choney and δ 13Cprotein of these samples were from −28.833 to −10.886 ‰ and −28.327 to −21.632 ‰, respectively. Interestingly, a very strong correlation was observed between δ 13Choney value and δ 13Cprotein value of pure honey. Moreover, δ 2H values of the honey samples were calculated between −156.725 and −170.029 ‰. The comprehensive procedure has advantages over existing methods in terms of time and sensitivity.
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