Hydrogels Loaded with Methylene Blue: Sorption-Desorption and Antimicrobial Photoactivation Study

O Nadtoka,P Virych,N Kutsevol

doi:10.1155/2020/9875290

O Nadtoka, P Virych + Show 1 more

Open Access

https://doi.org/10.1155/2020/9875290

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Abstract

Cross-linked dextran-graft-polyacrylamide hydrogels with different cross-linking density loaded with methylene blue was prepared as light-activated antimicrobial materials. Sorption/desorption properties of hydrogels were studied by estimating the change in the absorption maximum of MB solutions. The sorption/desorption rate of MB into/out of hydrogel depends on the hydrogel structure. The antibacterial properties of the light-irradiated materials were tested against Staphylococcus aureus. It was shown that irradiation of hydrogels in the presence of methylene blue was able to eliminate the bacteria strains.

Highlights

Bacterial infection usually occurs in burns, wounds, and lots of surgical operations [1]
Light-activated antimicrobial agents when excited with light of an appropriate wavelength, namely, with red light, generate singlet oxygen and/or radicals which are highly cytotoxic to bacteria [4, 5]
Hybrid cross-linked hydrogel dextran-graftedpolyacrylamide with different cross-linking density was synthesized as promising materials of a new generation for biomedical application

Summary

Introduction

Bacterial infection usually occurs in burns, wounds, and lots of surgical operations [1]. Microorganisms play an essential role in the development and maintenance of pathologies, and their removal during the wound healing or biomechanical preparation is important to the success of treatment. To eliminate or reduce bacterial colonization, methods that included the use of high-dose antibiotic were used. The antibiotic is largely successful but can lead to the development of antibiotic-resistant strains of bacteria. One novel approach for the elimination of bacteria on different surfaces is to use a light-activated antimicrobial agent (LAAA) [2, 3]. Light-activated antimicrobial agents when excited with light of an appropriate wavelength, namely, with red light, generate singlet oxygen and/or radicals which are highly cytotoxic to bacteria [4, 5]. The use of LAAAs to treat or prevent infectious diseases is known as photodynamic therapy (PDT) [6]

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