Abstract
Accurate identification and quantification of proteins in a solution using nanopores is technologically challenging in part because of the large fraction of missed translocation events due to short event times and limitations of conventional current amplifiers. Previously, we have shown that a nanopore interfaced with PEG(1000)-DMA hydrogel significantly enhances protein residence time inside the nanopore, reducing the number of missed events. (Acharya, ACS Sensors 2020) Following up on this work, we also have demonstrated the sensitivity of the hydrogel-backed nanopores to measure unlabeled proteins as small as 5.5 kD in size and 10 fM in concentration.
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