Abstract
Biotechnological processes involving the presence of microorganisms are realized by using various types of stirred tanks or laboratory-scale dual-impeller commercial bioreactor. Hydrodynamics and mass transfer rate are crucial parameters describing the functionality and efficiency of bioreactors. Both parameters strictly depend on mixing applied during bioprocesses conducted in bioreactors. Establishing optimum hydrodynamics conditions for the realized process with microorganisms maximizes the yield of desired products. Therefore, our main objective was to analyze and define the main operational hydrodynamic parameters (including flow field, power consumption, mixing time, and mixing energy) and mass transfer process (in this case, gas–liquid transfer) of two different commercial bioreactors (BioFlo® 115 and BioFlo® 415). The obtained results are allowed using mathematical relationships to describe the analyzed processes that can be used to predict the mixing process and mass transfer ratio in BioFlo® bioreactors. The proposed correlations may be applied for the design of a scaled-up or scaled-down bioreactors.
Highlights
Bioreactors are defined as facilities that enable the efficient operation of microbiological processes by controlling culture parameters and managing its optimal conditions while limiting the possibilities of its contamination [1,2] Bioreactors are the main part of any biotechnological processes in which living microorganisms are applied to produce a wide range of desired bioproducts with maximal efficiency
Sterile conditions should be maintained for pure culture systems; thermal sterilization is commonly applied; chemical sterilization is preferred for heat-sensitive equipment; other methods of sterilization: radiation by UV or X-rays; application of membrane filters; sterilization formation of the optimal morphology of the living microorganisms; elimination of contamination; In general, the industrial production may be carried out using bioreactors divided into three groups: (i) non-stirred and non-aerated systems (~76% of all bioreactors); (ii) non-stirred but aerated systems (~11% of all bioreactors); (iii) stirred and aerated systems (~13% of all bioreactors)
This paper focuses on the performance of two BioFlo® systems (BioFlo® 115 and BioFlo® 415), considering both mixing properties and mass transfer ratio of these multi-impeller systems
Summary
Bioreactors are defined as facilities that enable the efficient operation of microbiological processes by controlling culture parameters and managing its optimal conditions while limiting the possibilities of its contamination [1,2] Bioreactors are the main part of any biotechnological processes in which living microorganisms are applied to produce a wide range of desired bioproducts with maximal efficiency. Oxygen mass transfer characteristics for various twin- and single-impeller systems (e.g., Rushton turbine, pitched 4-, and 2-blade impellers) were studied by Karimi et al [18], while the power consumption and the mixing time for the non-baffled agitated vessel with a double impeller were measured by Hiraoka et al [19], who later discussed the best set-up position and the combination of impellers. We aimed at analyzing the power consumption, mixing time and energy (or homogenization energy), and mass transfer coefficient for the BioFlo® 115 and BioFlo® 415 bioreactors used under gassed and ungassed conditions
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
