Abstract
Orbitally shaken bioreactors (OSRs) of nominal volume from 50 mL to 2’000 L have been developed for the cultivation of suspension-adapted mammalian cells. Here we study the hydrodynamics of OSRs for mammalian cells. The results are expected to allow the determination of key parameters for cell cultivation conditions and will facilitate the scale-up of OSRs.
Highlights
Shaken bioreactors (OSRs) of nominal volume from 50 mL to 2’000 L have been developed for the cultivation of suspension-adapted mammalian cells
According to the Computational Fluid Dynamics (CFD) simulations of a 1-L bottle at 110 rpm, the maximal shear stress value was situated at the tip of the wave and was about 0.075 Pa (Figure 1a)
The maximal values of shear stress were always situated at the tip of the wave independently of the agitation rate
Summary
Shaken bioreactors (OSRs) of nominal volume from 50 mL to 2’000 L have been developed for the cultivation of suspension-adapted mammalian cells. We study the hydrodynamics of OSRs for mammalian cells.
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