Abstract
Sedimentation equilibrium and sedimentation velocity measurements were carried out on cytochrome P-450 LM2 from phenobarbital-treated rabbit liver and on cytochrome P-450 LM4 from 5,6-benzoflavone-treated rabbit liver in the presence of the nonionic detergent 1-O- n - octyl-β- D- glucopyranoside . P-450 LM2 was monomeric with a molecular weight of 48,800 and a Stokes radius of 3.1 nm in 7 g/l detergent and P-450 LM4 was monomeric with a molecular weight of 49,800 and a Stokes radius of 2.6 nm at 5 g/l detergent. Both particles were spherical in shape under these conditions. Neither cytochrome was irreversibly denatured at these detergent concentrations as indicated by the ability to form substantial amounts (>60%) of the CO adduct with an absorption maximum at 451 nm (P-450 LM2) or 448 nm (P-450 LM4) when diluted into detergent-free buffer containing CO and sodium dithionite.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
