Hydrochloric acid pretreated agro wastes as carbon source on CM-cellulases production by Aspergillus niger

Abstract

Maximum cell growth 0.936g/100ml of Aspergillus niger was observed at pH 4.11 in hydrolyzed rice husk used as carbon source. Aspergillus niger was isolated from the soil of Khairpur. The collection and utilization of suitable Agro wastes used as a carbon source for cellulases production by fungi desires optimized fermentation process. Five agricultural wastes were considered for cellulolytic enzyme production using pretreatment methods acid. Acid pretreatment was found to be the most efficient method with higher enzyme production. Using this cheap and renewable residue, for cellulolytic enzyme production by Aspergillus niger boosts its economic value which is not comparable with its current use as animal feed. Agro wastes such as sugarcane peelar bagasse, sugarcane bagasse, banana fruit stalk, sorghum husk and rice husk were hydrolyzed with 0.6N HCl. Rice husk was found better substrate in comparison to other Agro wastes for the growth of Aspergillus niger and cellulases production. Maximum activity of cellulases was noted 4.811 (units/ml). The Cellobiase and salicinase maximum production 4.717 and 4.742 units per ml obtained at 240 hours respectively.