Hydrocarboniclastica marina gen. nov., sp. nov., a marine hydrocarbonoclastic bacterium isolated from an in situ enriched hydrocarbon-degrading consortium in sea sediment.

Abstract

A Gram-stain-negative, motile, non-spore-forming, aerobic and rod-shaped bacterial strain, Soil36-7T, was isolated from an in situ enriched hydrocarbon-degrading consortium in South China Sea sediment. Strain Soil36-7T grew at 4-40 °C (optimum 28-32 °C), at pH 5-10 (pH 7-8) and in the presence of 1-12 % (w/v) NaCl (3-6 %). Phylogenetic analyses based on 16S rRNA gene sequences and a genome-based approach using UBCGs (up-to-date bacterial core genes) showed Soil36-7T formed a distinct branching lineage within the family Alteromonadaceae. 16S rRNA gene sequence similarity was 92.9, 92.1 and >88.3 % between strain Soil36-7T and the type species of the genera Marinobacter, Tamilnaduibacter and the other genera of the family Alteromonadaceae, respectively. The major fatty acids in Soil36-7T were C16 : 0, C16 : 1ω6/7c, C16 : 0 10-methyl, C18 : 1ω7c, C12 : 0 and C18 : 0. The predominant respiratory quinone was Q-9, with a minor amount of Q-10 (3.5 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and various unidentified glycolipids, phospholipids, aminophospholipids and other polar lipids. The DNA G+C content was 57.9 mol%. On the basis of phylogenetic, genomic, phenotypic and chemotaxanomic characteristics, strain Soil36-7T could be classified as representing a novel species of a new genus within the family Alteromonadaceae, for which the name Hydrocarboniclastica marina gen. nov., sp. nov. is proposed. The type strain of the type species is Soil36-7T (=MCCC 1A12105T=KCTC 62334T).