Hydrocarbon-Degrading Bacteria’s Selection from Soil Contaminated Engine Oil Waste of South Lampung

Abstract

Waste containing hydrocarbon compounds is classified as hazardous and toxic waste. Waste management must be done properly to prevent environmental pollution that ultimately has an impact on environmental damage. Engine oil is one of the hydrocarbon compound products that are widely used by motor vehicles. Waste engine oil from automotive repair shops has not been properly managed because Waste engine oil is often thrown away on the ground around the workshop. This condition has been going on for a long time due to soil contamination by waste engine oil. Thus, most microbes cannot live in polluted soils. Isolation of microorganisms in polluted soils is carried out to obtain bacteria that can grow in hydrocarbon-contaminated soils and have the potential to degrade hydrocarbon compounds. After isolation bacteria from two selected locations (Way Hui and Way Kandis), this research got four bacteria. These four isolates were selected by growing on selective media which added with engine oil with a concentration of 2% for 7 days. selection is done by counting the population of each isolate that lives in the media. Bacteria population was calculated on t1 (day 1), t4 (day 4) and on t7 (day 7). In addition, the selection is also done by calculating TPH (Total Petroleum Hydrocarbon) at t1 and t7. From these stages, it got one isolate (B7) which showed the highest results in population and three isolates showed the highest TPH decreasing (B1, B6 and B7).