Abstract
Contamination by petroleum hydrocarbons causes serious dangers to human health and the environment, whether by accidental or chronic contamination. Due to the large flow of ships, the commercial harbor of Oran is subject to pollution particularly by polycyclic aromatic hydrocarbons. For that, bioremediation by indigenous microorganisms is the most important method to eliminate or decrease this contamination. In the present paper, hydrocarbon-degrading bacterium strain SP57N has been studied, newly isolated from contaminated marine sediments and sea water from the harbor of Oran (Northwestern-Algeria), using of Bushnell-Hass salt medium (BHSM). The strain SP57N was Gram-negative, oxidase negative, catalase negative, motile, Rod-shaped bacteria, identified molecularly as Pseudomonas mendocina based on partial 16S rDNA gene sequence analysis, using the BLAST program on National Centre for Biotechnology Information (NCBI) and the EzBioCloud 16S rDNA databases. This isolate could growth on high concentrations of crude oil (up to 10 %, v/v). The effects of some culture conditions such as temperature, NaCl concentration and pH on growth rate of strain SP57N on crude oil as the sole carbon and energy source were studied. In addition, growth kinetic of this isolate on crude oil during 20 days of culture at 140 rpm, under optimal culture conditions was considered. The results showed maximum growth rate at temperature 25°C, 3% (w/v) of NaCl concentration and pH 7. Results of growth kinetic on crude oil as sole carbon and energy source showed that the stationary phase was attained at day 12. Thus, Pseudomonas mendocina SP57N had effectively hydrocarbon-degrading potential, and could be used as an efficacy degrader to initiate a biological eco-friendly method for the bioremediation of the hydrocarbon pollution on the port of Oran, and marine environment.
Highlights
Marine environments harbour a constant microbial seed that can be shaped by changes in environmental conditions including contamination by petroleum components
Analysis of the continuous sequence determined of 928 bp (GenBank ID: MK825733) by the BLASTn alignment program, on the National Centre for Biotechnology Information (NCBI) database, and on the EzBiocloud database, reveals the belonging of the strain SP57N to the species Pseudomonas mendocina
The bacterial stain SP57N was grown on 2% (v/v) of crude oil as sole carbon and energy source in Bushnell-Hass salt medium (BHSM) medium, for 20 days, on their optimal culture conditions (Temperature 25°C; pH 7; and 3% (w/v) of NaCl concentration)
Summary
Marine environments harbour a constant microbial seed that can be shaped by changes in environmental conditions including contamination by petroleum components. Oil spills are a major source of oil pollution in the marine environments, even though they are spilled in small but continuous discharges of hydrocarbons from transportation and recreational activities. Prokaryotic communities are well pre-adapted to oil pollution, and several microorganisms exposed to this xénobiotiques have developed an active degradation response (Acosta-González and Marqués 2016). In-situ bioremediation is considered as one of the most effective and sustainable means to clean up oilcontaminated sediments. They can be exploited more efficiently if their multifactorial environmental and pollution
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