Hydraulically-operated Thin-layer Sampler for Sampling Heterogeneous Water Columns

Abstract

A necessary condition for investigating microbial communities in chemocline zones of stratified waterbodies is high-precision sampling at a depth resolution of several centimeters. We have modified a multi-syringe sampler that is conventionally used by researchers for this purpose. The main distinctive feature of our sampler is that plungers are operated hydraulically rather than pneumatically and, thus, our sampler has the following advantages: (1) the plungers cannot move spontaneously, which ensures sampling adequacy, especially in deep-water sampling; (2) filling of the syringes with the sample can be monitored from a distance; (3) during storage, the internal volume of the syringe and the whole hydraulic system is aseptic (if 70% ethanol is used as a working fluid), which is important if samples are to be used for microbiological tests. (4) the problem of sticking plungers is minimized, even after extended storage. Vertical sampling of the chemocline zones of meromictic lakes in Southern Siberia (Russia, Khakasia) at a depth resolution of 5 cm proves that the sampler is very reliable and simple to use.