Hydration properties of lamellar and non-lamellar phases of phosphatidylcholine and phosphatidylethanolamine

Abstract

Two of the most common phospholipids in biological membranes are phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Over a wide range of temperatures the PCs found in biological membranes form lamellar (bilayer) phases when dispersed in excess water, whereas PEs form either lamellar or hexagonal phases depending on their hydrocarbon chain composition. This paper details the hydration properties of lamellar and hexagonal phases formed by PCs and PEs, focusing on the energetics of hydration of these phases. For the hexagonal phase, the energy of bending the lipid monolayer is a critical term, with other contributions arising from the energies of hydrating the lipid headgroups and filling voids in the interstices in the hydrocarbon region. For the lamellar phase of PC, the water content is determined by a balance between the attractive van der Waals pressure and repulsive hydration and entropic (steric) pressures. In the case of PE bilayers, recent experiments demonstrate the presence of an additional strong, short-range attractive interaction, possibly due to hydrogen-bonded water interactions between N + H 3 groups in one bilayer and the PO 4 − groups in the apposing bilayer. This additional attractive pressure causes apposing PE bilayers to adhere strongly and to imbibe considerably less water than PC bilayers.