Hydrangea serrata (Thunb.) Ser. Extract Attenuate UVB-Induced Photoaging through MAPK/AP-1 Inactivation in Human Skin Fibroblasts and Hairless Mice.

Hee-Soo Han,Hyoung Kim,Da-Bin Myung,Sun Lee,Hye Ahn,Kyung-Tae Lee,Ji-Sun Shin

doi:10.3390/nu11030533

Abstract

Skin photoaging is mainly caused by exposure to ultraviolet (UV) light, which increases expressions of matrix metalloproteinases (MMPs) and destroys collagen fibers, consequently inducing wrinkle formation. Nutritional factors have received scientific attention for use as agents for normal skin functions. The aim of this study was to investigate the effect of hot water extracts from the leaves of Hydrangea serrata (Thunb.) Ser. (WHS) against ultraviolet B (UVB)-induced skin photoaging and to elucidate the underlying molecular mechanisms in human foreskin fibroblasts (Hs68) and HR-1 hairless mice. WHS recovered UVB-reduced cell viability and ameliorated oxidative stress by inhibiting intracellular reactive oxygen species (ROS) generation in Hs68 cells. WHS rescued UVB-induced collagen degradation by suppressing MMP expression, and reduced the mRNA levels of inflammatory cytokines. These anti-photoaging activities of WHS were associated with inhibition of the activator protein 1 (AP-1), signal transduction and activation of transcription 1 (STAT1), and mitogen-activated protein kinase (MAPK) signaling pathways. Oral administration of WHS effectively alleviated dorsal skin from wrinkle formation, epidermal thickening, collagen degradation, and skin dehydration in HR-1 hairless mice exposed to UVB. Notably, WHS suppressed UVB activation of the AP-1 and MAPK signaling pathways in dorsal mouse skin tissues. Taken together, our data indicate that WHS prevents UVB-induced skin damage due to collagen degradation and MMP activation via inactivation of MAPK/AP-1 signaling pathway.

Highlights

The skin, as the most vulnerable organ of the human body, functions as the first line of defense protecting the body against toxic chemicals, infections, and ultraviolet (UV) radiation [1]
We found that matrix metalloproteinases (MMPs)-1/-3 production and mRNA
In accordance with the in vitro data, we examined the levels of MMP-1/-3 and pro-inflammatory cytokines

Summary

Introduction

The skin, as the most vulnerable organ of the human body, functions as the first line of defense protecting the body against toxic chemicals, infections, and ultraviolet (UV) radiation [1]. Excessive UVB irradiation penetrates the skin and generates intracellular reactive oxygen species (ROS), which results in cellular oxidative stress and prolonged skin inflammation [3] through activation of mitogen-activated protein kinase (MAPK), activator protein 1 (AP-1), nuclear factor kappa B (NF-κB)/p65, and signal transduction and activation of transcription (STAT) pathways [4]. Activation of these pathways results in overexpression of inflammatory cytokines like tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 [5]

Objectives

Methods

Results

Discussion

Conclusion