Abstract
Shake flask studies with a murine hybridoma cell line show drastically increased cell yields per mL of culture medium as function of passage number (“cell age”), whereas the maximum antibody concentrations at the end of batch growth decreased from 180 μg/mL to 75 μg/mL after 10 months of serial propagation in flasks. The results are presented in the light of differences in cell metabolism with glucose, lactate, ammonia, glutamine, and selected amino acids being monitored.
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