Abstract
In this protocol, hybridization is first performed in conventional aqueous solvents at a temperature well below the melting temperature, and the hybrids are then washed at higher stringency in buffers containing quaternary alkylammonium salts. TMACl is used with probes that are 14-50 nt in length, whereas TEACl is used with oligonucleotides that are 50-200 nt in length.
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