Hybridization of EcoRI chloroplast DNA fragments of [formula omitted] to pulse labeled RNA from different stages of chloroplast development

Abstract

Transcription of Euglena chloroplast DNA was visualized by autoradiography of hybrids formed between electrophoretically separated EcoRI endonuclease fragments and [ 32P]-pulse labeled RNA prepared from cells at different stages of chloroplast development. The RNA was hybridized to Southern imprints of EcoRI chloroplast DNA digests. The EcoRI chloroplast DNA fragments were categorized by their ability to hybridize the different pulse labeled RNA's. Some fragments were transcribed: 1. both in the dark and throughout chloroplast development, 2. predominantly in the light, 3. in the dark and for only a brief period of time after transfer of the cells to the light. Other fragments were probably not transcribed.