Abstract
We investigated the relationship between the 19-×50-nm single-stranded RNA (ssRNA) mushroom bacilliform virus (MBV) and the double-stranded RNAs (dsRNAs) associated with La France disease of Agaricus bisporus. Agarose gel electrophoresis under formaldehyde-denaturing conditions revealed that the MBV genome was composed of a single RNA molecule of 4.4 kb. In northern analyses, a 1,4-kb cloned complementary DNA (cDNA) to MBV RNA hybridized to the full-length genomic RNA in purified virus preparations and total RNA fractions from diseased mushrooms (...)
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