Abstract
Tandem mass spectrometric analyses of peptides using hybrid sector/quadrupole instruments may incorporate low energy collisionally activated decomposition (CAD), in the rf-only multipole region, or high energy CAD, in a field-free region of the sector component. Low energy CAD of peptides is strongly influenced by the site of charge and the extended time scale is conducive to the observation of prominent rearrangement processes. High energy CAD provides data of complementary value (though such data are recorded with poorer sensitivity than may be achieved with four-sector instruments). Sequential MS (MS3 and MS4) may be performed with hybrid instruments, permitting the elucidation, for example, of peptide fragmentation pathways.
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