Hybrid magnetic field system with helmholtz coils and magnets for real-time circulating tumor cell separation

Abstract

Extensive research is currently underway to devise effective strategies for the isolation of circulating tumor cells (CTCs) from blood samples. The utilization of magnetic particles and magnet as a means for this separation process is garnering increased attention within this field. However, when utilizing fixed magnets to capture cancer cells with attached magnetic particles, the inability to control the magnetic field may result in cellular damage due to the strong magnetic field compressing the magnetic particles. In this study, we developed a hybrid system that leverages electromagnetic coils to modulate the strength of the magnetic field from the magnets within a microfluidic channel, enabling real-time control of cancer cells with attached magnetic particles.Through the strategic application of electromagnetic coils, we achieved precise control over the magnet’s position, thereby enabling real-time observation of the cancer cell within the microfluidic channel. Additionally, this approach facilitated the release of captured cancer cells from the magnet. The isolated cells could then be cultured to assess individualized drug responses, and evaluate their reactivity to the treatment. Through the implementation of this novel technique, we anticipate that the real-time capture and analysis of CTCs from blood samples will enable more efficient and effective diagnostics and treatment monitoring, ultimately improving patient outcomes.