Abstract
We report on the design and fabrication of a hybrid sensor that integrates transmission-mode localized surface plasmonic resonance (LSPR) into a quartz crystal microbalance (QCM) for studying biochemical surface reactions. The coupling of LSPR nanostructures and a QCM allows optical spectra and QCM resonant frequency shifts to be recorded simultaneously and analyzed in real time for a given surface adsorption process. This integration simplifies the conventional combination of SPR and QCM and has the potential to be miniaturized for application in point-of-care (POC) diagnostics. The influence of antibody-antigen recognition effect on both the QCM and LSPR has been analyzed and discussed.
Highlights
Optical and mechanically oscillating sensing techniques have applications in in-situ, label free sensing and analysis of chemical and biological binding reactions (Mayer and Hafner, 2011; Arnau, 2008)
The sensitivity of Localized surface plasmon resonance (LSPR) is comparable to surface plasmonic resonance (SPR) in biomolecule binding reactions.(Willets and Van Duyne, 2007) Both LSPR sensor and Quartz crystal microbalance (QCM) have great potential regarding to point-of-care (POC) tests. (Aćimović et al, 2014; Yuan and Han, 2015) in this paper, we report the design of a hybrid sensor that integrates transmission-mode LSPR with a QCM which can be useful for the analysis of immunological reactions, film structures and other biological sorption reactions
The linear range of the sensor is between 10–50 μg/mL and the limit of detection (LOD) is 5 μg/mL for QCM and 1.88 μg/mL for LSPR measurements
Summary
Optical and mechanically oscillating sensing techniques have applications in in-situ, label free sensing and analysis of chemical and biological binding reactions (Mayer and Hafner, 2011; Arnau, 2008). Optical sensing techniques, such as surface plasmonic resonance (SPR), typically measure the change in the refractive index arising from the molecular adsorption on the metal surface measures the molecular mass of thin films (Zong et al, 2008). The sensitivity of LSPR is comparable to SPR in biomolecule binding reactions.(Willets and Van Duyne, 2007) Both LSPR sensor and QCM have great potential regarding to point-of-care (POC) tests. (Aćimović et al, 2014; Yuan and Han, 2015) in this paper, we report the design of a hybrid sensor that integrates transmission-mode LSPR with a QCM which can be useful for the analysis of immunological reactions, film structures and other biological sorption reactions
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