Abstract
Glycine max cultivars Lee68, Nannong 1138-2, and Nannong 8831 were used as the female parents, and hybrid lines (F5) 4,111, 4,076 (N23674 × BB52), 3,060 (Lee68 × N23227), and 185 (Jackson × BB52) that selected for salt tolerance generation by generation from the cross combination of G. max and G. soja were used as the male parents, 11 (A–K) backcrosses or three-way crosses were designed and 213 single hybrids were harvested. The optimized soybean simple sequence repeat (SSR)–polymerase chain reaction (PCR) system was used to analyze the SSR polymorphism of above parental lines and get the parental co-dominant SSR markers for hybrid identification, and in which 30 true hybrids were gained. The true hybrids (G1, G3, G9, G12, G13, G16) of G cross combination were chosen as the representative for the salt tolerance test, and the results showed that, as exposed to salt stress, the seedlings of G9 line displayed higher salt tolerant coefficient, relative growth rate, and dry matter accumulation, when compared with their female parent Nannong 1138-2, and even performed equally strong salt tolerance as the male parent 3,060. It provides a feasible method of the combination of molecular SSR markers and simple physiological parameters to identify the true hybrids of G. max and G. soja, and to innovate the salt-tolerant soybean germplasms.
Highlights
Salinity is one of the major abiotic stresses that adversely affect crop productivity and quality (Chinnusamy, Jagendorf & Zhu, 2005; Shrivastava & Kumar, 2015)
The randomly selected 24 pairs of primers were used to verify the stability of the system, and showed that all the primers could be all amplified with polymorphic bands (Fig. S1), indicating that this system could be used for soybean genetic diversity of simple sequence repeat (SSR) markers
True hybrid identification of different soybean cross In this study, 18 pairs of SSR primers were randomly selected in the soybean 20 linkage groups to screen the polymorphic SSR markers among seven parents of 11 combinations of backcrosses or three-way crosses (A–K)
Summary
Salinity is one of the major abiotic stresses that adversely affect crop productivity and quality (Chinnusamy, Jagendorf & Zhu, 2005; Shrivastava & Kumar, 2015). 22% of the world’s agricultural lands are affected by salinity (Bhatnagar-Mathur, Vadez & Sharma, 2008; Machado & Serralheiro, 2017). It is reported that more than 800 million hectares of land throughout the world, which account for more than 6% of the world’s total land areas, have been reported to be affected by salinity (Munns & Tester, 2008). In China, there are about 36 million hectares saline lands. What is worthy to be highly worried is that, the saline lands are expanding with the industry modernization, the increase of irrigated agrarian lands, and greenhouse for vegetables and flowers in agriculture (Zhang et al, 2011).
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