Hybrid full-pose parameter calibration of a freeform illuminator for Fourier ptychographic microscopy.

Abstract

As a typical computational method, Fourier ptychographic microscopy (FPM) can realize high spatial resolution and quantitative phase imaging while preserving the large field of view with a low numerical aperture (NA) objective. A programmable light-emitting diode (LED) array is used as a typical illuminator in an FPM system, and the illumination parameters of each LED element are crucial to the success of the FPM reconstruction algorithm. Compared with LED arrays arranged in rectangular arrays, LED arrays with special structures such as domes or rings can effectively improve FPM imaging results and imaging efficiency. As a trade-off, their calibration difficulty is greatly increased due to the lack of geometric constraints of rectangular arrays. In this paper, we propose an effective hybrid full-pose parameter calibration method for freeform LED array illuminators, combining stereoscopic 3D imaging techniques and the geometric constraints of the microscopic platform. First, a stereovision system is used to obtain the accurate 3D position of each LED element of the freeform illuminator and to construct a rigid 3D coordinate LED array system. Then, calibration between the coordinate system of the LED array and that of the optical imaging component is realized according to the geometric features of the brightfield-to-darkfield edges. Finally, we verify the feasibility and effectiveness of the proposed method through full-pose parameter calibration of LED arrays with different arrangement rules.