Hybrid capsules as efficient non-viral vector for gene delivery into cells

Abstract

Advances in the field of nanotechnology make it possible to employ nano- and microparticles as delivery carriers of genetic materials. In this study, hollow capsules of different composition were used as non-viral vectors to deliver plasmids into human cervical carcinoma cells (HeLa). Plasmids were encoded to express green fluorescent protein (eGFP) inside cells. Hybrid capsules were produced from degradable polyelectrolytes with an outer silica layer. Capsules were characterized with scanning electron and bright field microscopy. Successful cell transfection was verified with the confocal laser scanning microscope (CLSM).