Hybrid Beads Bearing Immobilized Bacteria as Advanced Means for the Removal of Acid Blue 93 Dye

Teodor Sandu,Tanta Verona Iordache,Marinela Victoria Dumitru,Iuliana Raut,Andrei Sarbu,Maria Luiza Jecu,Mariana Calin,Elvira Alexandrescu

doi:10.37358/mp.20.3.5387

Abstract

This paper describes the preparation of three- component hybrid copolymer beads, with water purification features. These newly developed hybrid beads were prepared starting from a mixture of poly(acrylonitrile- co- methacrylic acid) (PAN-co-PMAA), polyvinyl alcohol (PVA) and magnetite (Fe3O4), respectively. The preparation itself involved a Wet Phase Inversion (WPI) process. A Pseudomonas sp. strain was immobilized onto previously mentioned beads, before and after activation of the beads surface with glutardialdehyde, and afterwards used for the amendment of simulated water bearing an azo- blue dye, i.e. Acid Blue 93. In order to highlight the immobilization of Pseudomonas sp. strain, FTIR spectra and TGA results were recorded. CFU measurements as well as SEM images further provided evidence towards the occurrence of immobilization. The biodegradation studies of Acid Blue 93were carried out by means of UV spectroscopy at various contact times (24; 72 and 144 h) of the hybrid beads with the targeted dye.

Highlights

The wide use of dyes for fibers and paints results in a large amount of wastewaters
In order to confirm the immobilization of bacteria on the hybrid beads, Fourier Transform Infrared (FTIR) spectra were recorded, before and after immobilization of bacterial cells
For the unmodified beads, the immobilization of bacteria was only physical and the bacteria layer was washed with water

Summary

Introduction

The wide use of dyes for fibers and paints results in a large amount of wastewaters. These wastewaters pose great threat to the environment and human health especially when they reach surface and afterwards groundwaters [1]. A great deal of research was conducted, seeking advanced means of reducing the impact of such dyes. One negative effect of dyes may be attributed to their slow biodegradability. Among the already known methods that were proven to be somewhat efficient for dyes removal, it is worth mentioning: adsorption, coagulation, photocatalytic oxidation, and biodegradation. All these approaches are highly energy-consuming and, their cost-efficiency is rather low

Methods

Results

Conclusion