Abstract
Variovorax paradoxus VAI-C was isolated due to its ability to utilize acyl-homoserine lactones (AHLs) as the sole source of carbon, energy, and nitrogen. Here, we present a hybrid assembly of the V. paradoxus VAI-C genome sequence, consisting of a primary chromosome, a secondary chromid, and a plasmid.
Highlights
Variovorax paradoxus VAI-C was previously isolated [1] from soil adjacent to the Bowen Science Building at the University of Iowa, based on its ability to utilize the quorumsensing signal molecule N-(3-oxohexanoyl)-L-homoserine lactone (3OC6-HSL; “Vibrio fischeri autoinducer-1”) as the sole source of carbon and energy
The same DNA sample with additional needle shearing was used to generate a 250- to 300-bp library with the Nextera DNA Flex library preparation kit (LPK), which was sequenced on the Illumina iSeq platform (2 Â 150 bp)
A total of 4,818,968 Illumina reads were obtained for V. paradoxus VAI-C FastQC v0.11.8 was used for quality assessment of these data [9], and trimming was performed in Trimmomatic v0.38.0 [10]
Summary
Variovorax paradoxus VAI-C was previously isolated [1] from soil adjacent to the Bowen Science Building at the University of Iowa, based on its ability to utilize the quorumsensing signal molecule N-(3-oxohexanoyl)-L-homoserine lactone (3OC6-HSL; “Vibrio fischeri autoinducer-1”) as the sole source of carbon and energy. The same DNA sample with additional needle shearing was used to generate a 250- to 300-bp library with the Nextera DNA Flex library preparation kit (LPK), which was sequenced on the Illumina iSeq platform (2 Â 150 bp). A total of 4,818,968 Illumina reads were obtained for V. paradoxus VAI-C (average read length, 132.20 6 30.34; coverage, 67.49Â) FastQC v0.11.8 was used for quality assessment of these data [9], and trimming was performed in Trimmomatic v0.38.0 [10]. Assemblies of V. paradoxus VAI-C were created using a hybrid approach in Unicycler v0.4.8.0 [11] on the North America Galaxy hub (http://usegalaxy.org) [12].
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