Hyaluronidase enzymolysis of hyaluronic acid fillers in different products

Abstract

Objective To study the process of hyaluronidase enzymolysis under different conditions and to provide the basis for clinical application. Methods The experiment of 15 hyaluronic acid products from 10 brands was conducted in vitro with 15 concentrations and 4 dosages of hyaluronidase at 37 ℃ and 24 ℃. The results of experiment were compared with the clinical observations. Results The duration of enzymolysis was inversely correlated with hyaluronidase concentration. The duration of enzymolysis process reduced 60% when equal hyaluronidase was used as compared with that when half hyaluronidase was used. The enzymolysis duration could be 24 folds in different products. The enzymolysis time was 2.9 folds at 24 ℃ [24 ℃ was (77.67±35.33) min], compared with that at 37 ℃[37 ℃ was (27.13±11.05) min]. It took much more time of enzymolysis in vivo than in vitro for the same product. Conclusions Enzymolysis efficiency is positively correlated with the concentration and dosage of enzyme and inversely associated with the particle size and viscosity. Key words: Hyaluronic acid; Hyaluronidase; Temperature; Time of enzymolysis