Abstract
1. 1. Hyaluronidase activity was assayed in aqueous extracts of venom sacs of the Hymenoptera: Vespa orientalis, Vespa crabro, Paravespula germanica, Paravespula vulgaris, Dolichovespula saxonica, Dolichovespula media and Polistes gallicus. 2. 2. The procedures consisted of (1) a turbidimetric measurement of the digestion products after incubation with hyaluronic acid or chondroitin sulfate and (2) of a colorimetric estimation of the N-acetylglucosamine-reacting material released from hyaluronic acid. 3. 3. All the extracts examined were found to possess activity in depolymerizing both hyaluronic acid and chondroitin sulfate. The capacity to degrade chondroitin sulfate was, however, much lower than that towards hyaluronic acid and in this respect the insect hyaluronidase resembles mammalian testicular hyaluronidase.
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