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Abstract
Hyaluronic acid (HA), a polysaccharide presents widely in the extracellular matrix of various animals, is used as an injectable gel material for regenerative medicine due to its biocompatibility. HA hydrogel can be produced from HA molecules via crosslinking. Physical crosslinking by noncovalent bonds is preferable rather than chemical crosslinking using a crosslinking agent to prevent unintended interactions in a biological environment and reduce inflammation caused by reaction by-products. In this study, HA was modified with two types of complementary single-stranded DNA obtained by digestion of pUC118 vector with the restriction enzyme Bsp1286I. When both of HA-DNAs were mixed, hybridized complementary strands acted as crosslinking points to form hydrogels. Shearing stress was applied to mix these DNA-conjugated HA solutions. As a result, a stiff hydrogel with an elastic modulus of about 100 Pa was obtained. The gel thus obtained would be facile to handle as an injectable gel that gains its structural properties from the shear stress applied through injection with a needle. In addition, DNA crosslinking points can be used for hybridization of the hydrogels with other biopolymers, cleavage with restriction enzymes and dissociation by thermal denaturation.
Highlights
Hydrogel is a three-dimensional network macromolecule with an ability to retain water molecules inside
We have focused on the advantages of interaction between DNA and fabricated Hyaluronic acid (HA) modified with two single-stranded DNA linkers with complementary sequences
Based on the integral ratio of peaks derived from the acetyl group of HA (1.6 ppm) and the methyl group of thymidine of DNA (1.3 ppm), the degree of DNA substitution (DS) was calculated to be 4.7% and 5.6% for HAOligo1 and HA-Oligo2, respectively
Summary
Hydrogel is a three-dimensional network macromolecule with an ability to retain water molecules inside Due to their excellent biocompatibility based on high water absorption and retention, hydrogels are widely used in medical applications, hygiene products and cosmetics. HA, a biopolymer composed of D-glucuronic acid and D-N-acetylglucosamine linked via alternating β-1,4 and β-1,3 glycosidic bonds, is expressed ubiquitously as a glycosaminoglycan in the extracellular matrix (ECM) of various animals [2]. Unlike the proteins such as collagen, whose sequences di er from species to species, HA has low antigenicity and is expected to be applied as an injectable gel
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