Abstract
In this work, we compared mRNA levels of Hyaluronan (HA) metabolism members and BRCA genes, known to be involved in the tumoral process, between tumor and non-tumor adjacent tissue and its correlation with previously proposed biomarkers (ER, PR, HER2 and KI67) in order to assess their value as a progression biomarkers. We show alteration in HA metabolism in colorectal but not breast cancer. However, we found a decrease in Hyaluronidase 1 HYAL1 levels in the breast but not colorectal cancer. We also show lower HA levels in tumor compared with normal tissue that could indicate a possible influence of tumor on its surrounding “normal” tissue. In both breast and colorectal cancer, CD44 and BRCA2 showed a strong positive correlation. Besides, our results show first indicators that qPCR of the analyzed genes could be used as an easy and low cost procedure for the evaluation of molecular markers we propose here.
Highlights
In order to establish the association between HA metabolism genes with previously proposed biomarkers, we first analyzed ER, PR, HER2 and KI67 in the tumor (TT) of the breast cancer patients and KI67 in TT of the colorectal cancer patients by immunohistochemistry (IHC)
We found a high amount of KI67 positive cells in all (42%–63%) but two (7% and 9%) colorectal cancer patients, while in breast cancer we found three low
All of the results were normalized to housekeeping gene GAPDH and shown as tumor In colorectal cancer samples, we found that none of the analyzed genes were consistently higher tissue (TT)orrelative tissue adjacent tumor lower in to TT non-tumor compared to non-tumor tissue adjacent to the tumor (NAT)
Summary
Cancer is one of the leading causes of death worldwide. It is estimated that in 2012 there were14.1 million new cases and 8.2 million deaths related to this disease. Cancer is one of the leading causes of death worldwide. It is estimated that in 2012 there were. 14.1 million new cases and 8.2 million deaths related to this disease. 70% of these cases occurred in developing countries. The most common types of cancer in men are lung, prostate, colorectal, stomach and liver, while breast, colon, lung, cervix and stomach cancer are the most. Biomedicines 2020, 8, 183 common among women [1]. Breast, cervical and colorectal cancer are among the most common cancer types in Latin America [2]. The cancer burden continues to be a global problem. Many health systems in underdeveloped and developing countries are not prepared to manage this problem due to low-income and as a consequence large numbers of cancer patients do not have access to quality diagnosis and treatment [3]
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