Huzhang Tongfeng Granule Improves Monosodium Urate-Induced Inflammation of Gouty Arthritis Rat Model by Downregulation of Cyr61 and Related Cytokines.

Mi Zhou,Ming Zhang,Yi Lu,Yi Ru,Yifei Wang,Xi Chen,Siting Chen,Xiaojie Ding,Liang Hua,Xin Li,Kan Ze,Bin Li

doi:10.1155/2020/9238797

Abstract

Objective Gouty arthritis (GA) is a noninfectious inflammatory disease characterized by self-limited and severe pain. Huzhang Tongfeng granule is one of the most effective traditional Chinese medicines in the treatment of acute GA. However, its effects on the inflammatory factors in the process of acute gout inflammation remain unknown. In the present study, we aimed to evaluate the effect of Huzhang Tongfeng granule on the expressions of Cyr61 and related inflammatory factors in both experimental gout models in vivo and in vitro. Methods Huzhang Tongfeng granule was provided by the pharmaceutical preparation room of Yueyang Hospital of Integrated Traditional Chinese and Western Medicine. The expressions of Cyr61, IL-1β, TNF-α, and IL-6 in monosodium urate- (MSU-) induced rat models and fibroblast-like synoviocytes (FLSs) were determined by RT-PCR, Western blotting analysis, ELISA, immunohistochemistry, and hematoxylin and eosin staining. Results Huzhang Tongfeng granule could downregulate the expressions of IL-1β, TNF-α, and IL-6 to some extent by inhibiting the expression of Cyr61. Conclusions Collectively, our findings indicated that Cyr61 was highly expressed in rat models of gout. By inhibiting the expression of Cyr61, Huzhang Tongfeng granule could partially attenuate the inflammation induced by MSU crystal.

Highlights

Acute gouty arthritis (GA) is a group of clinical syndromes caused by monosodium urate (MSU) crystal deposition on bone, joints, and subcutaneous tissues, which is the most common initial symptom of gout [1]
MSU crystals act as foreign bodies to induce the chemotactic aggregation of leukocytes. ey are transformed into a signal that strongly stimulates the toll-like receptors (TLRs) and NOD-like receptor family pyrin domain-containing-3 (NLRP3) inflammasome, which activate the innate immune signaling pathway [3] and produce a series of inflammatory factors
Histological observation showed that the synovial tissue structure of the normal control group was clear, the synovial cells were arranged in a single layer, the cell morphology was normal, and there was no inflammatory cell infiltration

Summary

Objective

Gouty arthritis (GA) is a noninfectious inflammatory disease characterized by self-limited and severe pain. Huzhang Tongfeng granule is one of the most effective traditional Chinese medicines in the treatment of acute GA. Its effects on the inflammatory factors in the process of acute gout inflammation remain unknown. We aimed to evaluate the effect of Huzhang Tongfeng granule on the expressions of Cyr and related inflammatory factors in both experimental gout models in vivo and in vitro. Huzhang Tongfeng granule was provided by the pharmaceutical preparation room of Yueyang Hospital of Integrated Traditional Chinese and Western Medicine. E expressions of Cyr, IL-1β, TNF-α, and IL-6 in monosodium urate- (MSU-) induced rat models and fibroblast-like synoviocytes (FLSs) were determined by RT-PCR, Western blotting analysis, ELISA, immunohistochemistry, and hematoxylin and eosin staining. By inhibiting the expression of Cyr, Huzhang Tongfeng granule could partially attenuate the inflammation induced by MSU crystal

Introduction

Materials and Methods

Results