Abstract
Plasma focus devices may arise as useful source to perform experiments aimed to study the effects of pulsed radiation on human cells in vitro. In the present work, a table top hundred joules plasma focus device, namely “PF-400J”, was adapted to irradiate colorectal cancer cell line, DLD-1. For pulsed x-rays, the doses (energy absorbed per unit mass, measured in Gy) were measured using thermoluminescence detectors (TLD-100 dosimeters). The neutron fluence and the average energy were used to estimate the pulsed neutron doses. Fifty pulses of x-rays (0.12 Gy) and fifty pulses of neutrons (3.5 μGy) were used to irradiate the cancer cells. Irradiation-induced DNA damage and cell death were assessed at different time points after irradiation. Cell death was observed using pulsed neutron irradiation, at ultralow doses. Our results indicate that the PF-400J can be used for in vitro assessment of the effect of pulsed radiation in cancer cell research.
Highlights
Hundred joules plasma focus device as a potential pulsed source for in vitro cancer cell irradiation
A plasma focus (PF) device consists in a central electrode that is partially covered by an insulator
The plasma dynamics in the PF devices is categorized in various phases
Summary
Hundred joules plasma focus device as a potential pulsed source for in vitro cancer cell irradiation. The radiation emitted from the PF devices have been applied widely in material science and nanotechnology[23] and recently in biology.[18,19,24] In this letter, the pulsed x-ray radiation and pulsed neutron bursts are used to irradiate the cancer cell lines during in vitro experiments. Both pulsed x-rays in low dose regime and pulsed neutrons in ultralow dose regime are used to irradiate the colorectal cancer cell DLD-1.
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