Humus accumulation, humification, and humic acid composition in soils of two post-mining chronosequences after coal mining

Abstract

To assess how the rates of humus formation and humification are affected by land use and age of ecosystems, this study investigated soil development in two post-mining chronosequences (spoil heaps formed from open-cast coal mining near Sokolov, Czech Republic). The following characteristics were measured: content, composition, and properties of humic acids; organic carbon (C) and total nitrogen (N) contents; pH; and amorphous iron content. Two chronosequences were studied. One consisted of unreclaimed spontaneously revegetated spoils (3, 12, 20, and 40 years old). The other consisted of heaps that were reclaimed by planting alder in graded heaps (7, 15, 20, 30, and 40 years old). Humus and iron contents as well as pH were determined, and humic acids were extracted for detailed chemical analyses (C, H, N, 13C-NMR, and pyrolysis mass-spectrometry). C and N accumulated faster in the reclaimed sites than in the unreclaimed sites; organic matter accumulation results in the decrease of pH in all soils and in spontaneous sites also increase of amorphous iron content. Humic acids (HA) and fulvic acids (FA) increased with the site age in both chronosequences. The CHA/CFA was higher in the reclaimed soils than in the unreclaimed soils, and the CHA/CFA ratio increased with age in the unreclaimed soils. Humic acid aromaticity was higher in the reclaimed site than in the unreclaimed sites but increased with the age of unreclaimed sites. H and O content decreased with age of reclaimed soils. Humification led to an increase in HA caloricity in the reclaimed sites but not in unreclaimed sites because of the influence of residual wax-type substances in the unreclaimed sites. Degree of HA oxidation (ω) for the reclaimed plots was similar to that in typical zonal soils but was highly variable in unreclaimed soils. Relative to spontaneous revegetation in the unreclaimed sites, reclamation increased the rates of humus accumulation, humification, and humus acid transformations. The differences between reclaimed and unreclaimed sites, however, decreased with site age and were very small in 40-year-old sites. These differences correspond to the rapid colonization of the reclaimed sites and the slow colonization of the unreclaimed sites by soil biota.