Abstract
The haemolymph plasma of adult desert locust, Schistocerca gregaria was analyzed in order to detect the induction of antibacterial activity following injection with a sublethal dose of Bacillus thuringiensis israelensis (Bti). A slight activity was demonstrated in the normal locust plasma, while a significant increase of this activity was appeared in both control and immune plasma, when tested by the agar-disk diffusion method. The maximum activity was attained after 24 h post-injection. Analysis of proteins by SDS polyacrylamide gel electrophoresis showed disappearance of some protein bands and appearance of other new ones in the injected adult locusts. Some of these new proteins may affect adult immunity. The phenoloxidase (PO) activity was significantly higher at 6 and 12 h after injection with Bti, but at 24 and 48 h, the activity was significantly lower than that of the controls. However, no significant changes in lysozyme activity were detected at all time intervals post-injection compared with the control insects.
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