Abstract
Background and objective: Serological test has become an important tool in the diagnosis of TB cases. This study focused on the analysis and comparison of antibody response to two Mycobacterium tuberculosis (MTB) antigens namely Ag85 complex and culture filtrate protein (CFP) in patients with tuberculosis. Antibody response to specific antigen was utilized as a diagnostic tool to detect active tuberculosis (TB) cases.Methods: Sera from 30 patients with active tuberculosis and 30 healthy individuals were tested by enzyme linked immunosorbent assay (ELISA) for the presence of immunoglobulin (Ig) G and IgM antibodies against Ag85 complex and culture filtrate protein (CFP) antigens of MTB.Results: The mean OD values of serum IgM and IgG antibodies against Ag85 and CFP were significantly (p<0.0001) higher in patients than that of healthy control individuals. Among the 30 tuberculosis patients, anti-Ag85 complex IgM and IgG was positive in 66.7% and 70.0% patients respectively. The seropositive rate of anti-CFP IgM and IgG was 33.3% and 56.7% respectively. The sensitivity and specificity of anti Ag85 and anti-CFP IgM and IgG ranged from 60.0% to 96.7%.Conclusion: The study demonstrated that determination of IgM and IgG antibodies against Ag85 complex and CFP could be used as a serological marker for diagnosis of active tuberculosis.IMC J Med Sci 2016; 10(2): 53-57
Highlights
Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB) is one of the leading cause of morbidity and death in the world
Antibody response to Ag85 complex and culture filtrate protein (CFP) was determined in sera of 30 confirmed cases of tuberculosis and 30 healthy individuals
It was observed that mean OD values of serum IgM and IgG antibodies against Ag85 complex and CFP were significantly
Summary
Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB) is one of the leading cause of morbidity and death in the world. The detection of MTB-specific antibodies has become important diagnostic aid in the diagnosis of TB especially in smear-negative sputum cases [3]. It is important for pediatric and extra pulmonary tuberculosis and in those unable to produce sputum. This study focused on the analysis and comparison of antibody response to two Mycobacterium tuberculosis (MTB) antigens namely Ag85 complex and culture filtrate protein (CFP) in patients with tuberculosis. Antibody response to specific antigen was utilized as a diagnostic tool to detect active tuberculosis (TB) cases
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