Abstract
Introduction Humic acid was previously shown to enhance cutaneous wound healing and show antibacterial properties; however, it has not been used for wound healing in the oral cavity. Thus, the goal of this study was the evaluation of the effect of the humic acid on the healing of excisional wounds in an experimental rat study. Materials and Methods A circular wound on mid-palatal surfaces was made on a total of 77 Wistar rats by using a 3-mm biopsy punch under anesthesia. The animals were divided into 4 groups as baseline, saline control (0.09%), chlorhexidine gluconate (0.05%), and humic acid (80 mg/kg) and were treated with these materials for 7 days. Results The rats were observed for 3 weeks in order to track the wound closure rates. Both humic acid treatment and chlorhexidine gluconate treatment resulted in statistically significant enhanced rate of wound closure compared to the saline control on both the 1st and 2nd weeks of treatment. Humic acid treatment for the wounds in the palate resulted in enhanced recovery compared to not only saline control but also chlorhexidine gluconate treatment. Conclusion In this study, humic acid was shown to enhance healing of oral wounds for the first time in the literature. These findings indicate that humic acid can be used as an alternative to current treatment methods for oral wounds.
Highlights
Humic acid was previously shown to enhance cutaneous wound healing and show antibacterial properties; it has not been used for wound healing in the oral cavity
We have previously shown that humic acid prevents alveolar bone loss and reduce inflammation in rats [20]
These results suggest the possibility that these specific properties of humates may be useful in wound healing in the palate, currently, there is no evidence showing the effects of humates on wound healing in the oral cavity
Summary
Humic acid was previously shown to enhance cutaneous wound healing and show antibacterial properties; it has not been used for wound healing in the oral cavity. The rats were observed for 3 weeks in order to track the wound closure rates Both humic acid treatment and chlorhexidine gluconate treatment resulted in statistically significant enhanced rate of wound closure compared to the saline control on both the 1st and 2nd weeks of treatment. Humic acid treatment for the wounds in the palate resulted in enhanced recovery compared to saline control and chlorhexidine gluconate treatment. The wound area is protected against pathogens and the dead cells are removed, whereas, during proliferation phase, the cells that secrete extracellular matrix materials proliferate and secrete high amounts of fibrous extracellular matrix proteins in order to rapidly block and protect the wound area against pathogens [4] During remodeling phase, these fibrous blocking components are removed and more functional tissue is generated. Other than the antibacterial products that are already commercially used, few factors were previously shown to aid palatal wound healing [5,6,7]
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