Humic acid and fulvic acid facilitate the formation of vivianite and the transformation of cadmium via microbially-mediated iron reduction

Abstract

The effects of humic acids (HA) and fulvic acids (FA) on the fate of Cd in anaerobic environment upon microbial reduction of Cd-bearing ferrihydrite (Fh) with Geobacter metallireducens were investigated. The results showed that HA and FA could promote the reductive dissolution of Fh and the formation of vivianite. After incubation of 38 d, vivianite accounted for 47.19%, 59.22%, and 48.53% of total Fe in biological control batch (BCK), HA and FA batches (C/Fe molar ratio of 1.0), respectively, by Mössbauer spectroscopy analysis. In terms of Cd, HA and FA could promote the release of adsorbed Cd during the initial bioreduction process, but reassuringly, after 38 d the dissolved Cd with HA and FA addition batches were 0.58–0.91 and 0.99–1.08 times of the BCK, respectively. The proportions of residual Cd in HA batches were higher than FA and BCK batches, indicating that HA was better than FA in immobilizing Cd. This might be because the quinone groups in HA could act as electron shuttle. This study showed that HA facilitated the transformation of vivianite better than FA, and Cd can be stabilized by resorption or co-precipitation with vivianite, providing a theoretical support for the translocation of Cd in sediment-water interface.