Human Umbilical Cord Mesenchymal Stem Cells Inhibit the Function of Allogeneic Activated Vγ9Vδ2 T Lymphocytes In Vitro.

Xiaohuan Liu,Tianxiang Gong,Tingting Zhu,Hong Li,Qihong Wu,Ting Feng,Chongyang Shen,Qiang Li

doi:10.1155/2015/317801

Abstract

Background. Human umbilical cord mesenchymal stem cells (UC-MSCs) can regulate the function of immune cells. However, whether and how UC-MSCs can modulate the function of Vγ9Vδ2 T cells has not been fully understood. Methods. The PBMCs or Vγ9Vδ2 T cells were activated and expanded with pamidronate (PAM) and interleukin-2 (IL-2) with or without the presence UC-MSCs. The effects of UC-MSCs on the proliferation, cytokine expression, and cytotoxicity of Vγ9Vδ2 T cells were determined by flow cytometry. The effects of UC-MSCs on Fas-L, TRAIL-expressing Vγ9Vδ2 T cells, and Vγ9Vδ2 T cell apoptosis were determined by flow cytometry. Results. UC-MSCs inhibited Vγ9Vδ2 T cell proliferation in a dose-dependent but cell-contact independent manner. Coculture with UC-MSCs reduced the frequency of IFNγ+ but increased granzyme B+ Vγ9Vδ2 T cells. UC-MSCs inhibited the cytotoxicity of Vγ9Vδ2 T cells against influenza virus H1N1 infected A549 cells and also reduced the frequency of Fas-L+, TRAIL+ Vγ9Vδ2 T cells but failed to modulate the apoptosis of Vγ9Vδ2 T cells. Conclusions. These results indicated that UC-MSCs efficiently suppressed the proliferation and cytotoxicity of Vγ9Vδ2 T cells and modulated their cytokine production. Fas-L and TRAIL were involved in the regulation. Cell contact and apoptosis of Vγ9Vδ2 T cells were not necessary for the inhibition.

Highlights

Mesenchymal stem cells (MSCs) can spontaneously proliferate and differentiate varieties of cell types, including osteoblasts, chondrocytes, adipoblasts, skeletal myocytes, and tenocyte [1, 2]
We characterized the frequency of peripheral blood Vγ9Vδ2 T cells by flow cytometry analysis and found that peripheral blood Vγ9Vδ2 T cells accounted for 3%–8% of T cells
We examined the effect of umbilical cord mesenchymal stem cells (UC-MSCs) on the proliferation and cytotoxicity of Vγ9Vδ2 T cells as well as their cytokine and effector expression in vitro

Summary

Introduction

Mesenchymal stem cells (MSCs) can spontaneously proliferate and differentiate varieties of cell types, including osteoblasts, chondrocytes, adipoblasts, skeletal myocytes, and tenocyte [1, 2]. Human umbilical cord mesenchymal stem cells (UC-MSCs) can regulate the function of immune cells. The PBMCs or Vγ9Vδ2 T cells were activated and expanded with pamidronate (PAM) and interleukin-2 (IL-2) with or without the presence UCMSCs. The effects of UC-MSCs on the proliferation, cytokine expression, and cytotoxicity of Vγ9Vδ2 T cells were determined by flow cytometry. The effects of UC-MSCs on Fas-L, TRAIL-expressing Vγ9Vδ2 T cells, and Vγ9Vδ2 T cell apoptosis were determined by flow cytometry. UCMSCs inhibited the cytotoxicity of Vγ9Vδ2 T cells against influenza virus H1N1 infected A549 cells and reduced the frequency of Fas-L+, TRAIL+ Vγ9Vδ2 T cells but failed to modulate the apoptosis of Vγ9Vδ2 T cells. These results indicated that UC-MSCs efficiently suppressed the proliferation and cytotoxicity of Vγ9Vδ2 T cells and modulated their cytokine production. Cell contact and apoptosis of Vγ9Vδ2 T cells were not necessary for the inhibition

Methods

Results

Conclusion