Abstract
The aim of the present study was to investigate the effects of human umbilical cord blood-derived mesenchymal stem cell (HUCB-MSC) transplantation on the functional restoration of spinal cord injury (SCI). A total of 46 adult Wistar rats were randomly divided into three groups: Injury (n=15), control (n=15) and transplantation (n=16). A SCI model was established using the modified Allen’s method (vulnerating energy, 25 g/cm). The rats in the control and transplantation groups were injected at the site of the injury with physiological saline and HUCB-MSC suspension, respectively. At week one, two and four following treatment, the behavior of the rats was evaluated using the Basso, Beattie, Bresnahan locomotor rating scale. In addition, immunohistochemistry (IHC) was performed on samples from the rats that had been sacrificed four weeks subsequent to the treatment. Recovery of the spinal cord nerve function was identified to be significantly different at week two and four following treatment (P<0.05), and IHC identified that at week four following treatment novel nerve cells were being produced. Thus, transplantation of HUCB-MSCs promoted the recovery of the damaged function of spinal cord nerves in rats with SCI.
Highlights
Spinal cord injury (SCI) is a common type of severe trauma
human umbilical cord blood (HUCB)‐mesenchymal stem cells (MSCs) were effectively isolated and amplified in vitro, and growth was observed in the specialized MSC medium
Redundant MSCs were obtained through passaging, which may provide the experimental foundation for future investigations and clinical applications
Summary
Spinal cord injury (SCI) is a common type of severe trauma. Maximum recovery of spinal cord function has become increasingly studied for the treatment of SCI. It was previously hypothesized that following injury, the central nerve is not able to regenerate; recent studies have identified that by altering the local environment following SCI, the injured nerve axons are able to regenerate and partial functioning of the spinal cord can be restored [1,2]. Previous studies have identified that marrow mesenchymal stem cells (MSCs) can be differentiated into neural cells in vitro and in vivo via induction. This provides a novel method for the treatment of SCI and has been shown to be effective in certain clinical applications [16,17,18]. The aim of the current study was to identify a type of cell that is suitable for the treatment of SCI and to provide further experimental evidence for its clinical application
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