Abstract
Human umbilical cord blood (HUCB) is an important source of progenitor cells which are able to reconstitute hematopoiesis. The in vitro expansion of isolated CD34 positive cells should provide a sufficient number of hematopoietic cells for transplants in large reciplents and would increase vector-mediated gene transfer efficiency. In vitro expansion of progenitor cells requires the identification of the cytokine combinations which result in the greatest increase of committed cells, without exhausting the early progenitor pool. In this study we tested the effect of three colony stimulating-factor (CSF) combinations [SCF*+IL-6(CSF2); SCF*+IL-6+FL (CSF3); SCF*+IL-6+FL+IL-3 (CSF4)] on CD34+ cells isolated(MiniMACS, Miltenyi Biotec, Germany) from HUCB. CD34+ cells, ≥97% pure in all samples, were ≥97% CD33- (range 97-99%) and ≥97% CD38+ (range 97-99.8%). The absolute number of total cells and cell subsets, evaluated after 7 and 11 days of liquid cultures, showed the following median fold increase:Table
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