Abstract
AbstractSince pre-mRNA splicing is processed by a spliceosome that is a huge complex consists of RNAs and as many as 200 proteins, it is considered that minimum intron size is limited by molecular size of the spliceosome. To understand splicing mechanism, we investigate human intron size distribution using annotated transcriptome database, H-InvDB. Distribution of obtained intron length shows that there is a mode at 83 nt in length with 4049 transcripts and number of introns decreases drastically in shorter than 65 nt, where numbers of transcripts in each locus also decrease. However, some introns less than 65 nt are observed, both shows high Codon Adaptation Index (CAI) and also observed orthologous transcripts in other mammals. These suggests that there is a limitation in minimum length to spliced out in general splicing mechanism of human, but also unknown mechanism may be exist to splice small intron exceeding the limitation.
Highlights
Since pre-mRNA splicing is processed by a spliceosome that is a huge complex consists of RNAs and as many as 200 proteins, it is considered that minimum intron size is limited by molecular size of the spliceosome
We investigate human intron size distribution using annotated transcriptome database, H-InvDB
Distribution of obtained intron length shows that there is a mode at 83 bp in length with 4049 transcripts and number of introns decreases drastically in shorter than 65 bp, where numbers of transcripts in each locus decrease
Summary
Since pre-mRNA splicing is processed by a spliceosome that is a huge complex consists of RNAs and as many as 200 proteins, it is considered that minimum intron size is limited by molecular size of the spliceosome. We investigate human intron size distribution using annotated transcriptome database, H-InvDB. Distribution of obtained intron length shows that there is a mode at 83 bp in length with 4049 transcripts and number of introns decreases drastically in shorter than 65 bp, where numbers of transcripts in each locus decrease. Some introns less than 65 bp are observed, both shows high Codon Adaptation Index (CAI) and observed orthologous transcripts in other mammals. These suggests that there is a limitation in minimum length to spliced out in general splicing mechanism of human, and unknown mechanism may be exist to splice small intron exceeding the limitation
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