Abstract
A comparative kinetic study on the poly(gallic acid disulfide) (poly(DSGA)) inhibition of the iodide ion oxidation and on the 2-hydroxy-3,5-di-tert-butyl-N-phenylaniline (butaminophene) inhibition of 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation involving human thyroid peroxidase (hTPO) and horseradish peroxidase (HRP) was performed. The inhibition processes were characterized with the inhibition constantsK i and stoichiometric inhibition coefficientsf, indicating the number of radical particles perishing on one inhibitor molecule. In the case of poly(DSGA), theK i values for the I− oxidation were 0.60 and 0.04 µM, and the coefficientsf were 13.6 and 16.5 for hTPO and HRP, respectively, which evidences the regeneration and high effectiveness of the polymeric inhibitor. In the case of butaminophene, theK i values for TMB oxidation were 38 and 46 µM for hTPO and HRP, respectively. The coefficientsf were 1.33 and 1.47, respectively, to reveal that butaminophene does not regenerate. The inhibition mechanisms for I− and TMB oxidation involving the two peroxidases are discussed.
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