Human Skeletal Remains

The identification of missing persons and human remains is a worldwide problem which has been exacerbated with increased migrations and rampant human trafficking and smuggling cases. DNA typing and DNA databases are primary tools and resources used to help identify human remains and missing persons. The foundation of most, if not all, national DNA database systems, e.g., CODIS, is law enforcement identification. With such database systems, compliance with statutory and operational requirements is necessary to ensure the integrity of the databases. However, because of conditions in their homelands, relatives of missing persons at times may not trust the government and may be reluctant to contact a law enforcement agency, making it difficult to satisfy the law enforcement nexus necessary for entry into a national DNA database. A potential solution to increase the identification of unidentified human remains found within the USA, such as those that may be of foreign nationals, the University of North Texas Center for Human Identification (UNTCHI) has created a Humanitarian DNA Identification DNA Database (HDID) that enables family reference sample DNA profiles from non-US citizens to be compared with the DNA profiles from unidentified human remains within its local database system. This short communication describes the needs, basis, policies, and practices to inform the scientific, investigative, and legal communities and the public so that various entities may become aware and consider submitting family reference sample (FRS) profiles from foreign nationals for the purpose of searching against UNTCHI's HDID. It is our hope that by creating this HDID, another vehicle is available to support identification of human remains within the USA and to bring much needed answers to the family members of missing persons. The HDID will merge high forensic quality and best practices with the broader accessibility for non-US families to voluntarily donate DNA profiles for searching for missing loved ones.

The essential purpose of the forensic anthropological discipline is the identification of human remains when nearly or completely skeletonized remains are found and a standard soft tissue autopsy can no longer be performed. Identification of human skeletal remains is defined as the act of establishing an identity that permits an individual to be definable and recognizable. In archaeological and forensic contexts, identification involves the possibility to attribute a correct name to human remains. Different circumstances, such as those involve an unexplained natural death, homicide, accident or political, ethnic or religious violence, or mass disaster events, can require to establish 290the identity of a deceased individual. In all these situations, the joint use of physical and molecular methods can be effective in allowing the identification activity. This chapter will introduce the forensic anthropological activity as a fundamental tool in identification contexts of human skeletal remains and will present how a DNA analysis expert of highly degraded samples may be an important ally of physical anthropologist in challenging situations.

Investigative and policy impacts of forensic genetic genealogy in the identification of human remains

Heating Up Cold Cases: An Interview with Bruce Budowle on Human Identification

European and Northwest African Middle Pleistocene Hominids by F. Clark Howell of human skeletal remains from the Middle Pleistocene has always been one of the greatest gaps in human-paleontological knowledge. At THE SPARSE REPRESENTATION first, Southeastern Asia was unique in having provided remains from the Trinil beds in Java, but the signifi­ cance of this poorly preserved skull-cap was confirmed and greatly amplified by subsequent discoveries (Von Koenigswald 1940) of better preserved specimens at other localities of similar age, as well as in the still older Djetis beds. Still tfuly unique in all the world is the somewhat younger occupation site of Locality 1 Choukoutien, with its extraordinarily abundant, prob­ ably cannibalized, human remains in association with hearths, stone implements (Choukoutienian chopper/ chopping-tool complex), and remains of slaughtered animals. For many years, the only such find from the West was the enigmatic human mandible from the Grafenrain gravel pit at Mauer in the Rhineland. Then, within a few years in the mid-thirties, three additional speci­ mens came to light in western Europe (Steinheim), Britain (Swanscombe), and northwest Africa (Rabat, Morocco). In the last several years, further Middle Pleistocene human remains were found in northwest Africa, both in Algeria (Ternifine) and in Morocco (Sidi Abderrahman). All these discoveries (Fig. I) have excellent paleontological associations, and, in three cases (Swanscombe, Ternifine, Sidi Abderrahman), there are associated stone implements (Acheulean of various stages). However, no occupation site is yet F. CLARK HOWELL is Associate Professor of Anthropology, De­ partment of Anthropology, University of Chicago (Chicago, Illinois, U.S.A.). He was born in 1925, and educated at the University of Chicago (Ph.D., 1953). HOWELL has undertaken field and other studies on early man in Europe (1953,1956) and in Africa (1954,1957-58,1959), and made a recent brief visit to Israel (1959). He has published various papers on human paleontology, especially as regards the Neanderthal problem, and on early man and the Pleisto­ cene in general. Prior to submitting the present paper to CURRENT ANTHRO­ POLOGY, HOWELL sent it, for comment and criticism, to three colleagues, of whom W. W. Howells and Kenneth P. Oakley responded. The response was primarily commendatory, and except for HOWELL's addition of some paragraphs along lines recommended by Oakley, the paper stands as first written. Vol. I . No.3' Ma)' 1960 known from the Middle Pleistocene of Europe or Africa with in situ human skeletal remains, stone im· plements, and the bones of slaughtered animals. These human skeletal remains, taken in conjunction with those from the Middle Pleistocene of eastern and southern Asia, have an important bearing on interpre­ tations of the course of human evolution. The signifi­ cance of these discoveries has been obscured by the pre­ occupation of some human paleontologists with other human remains either suspected to be of Pleistocene antiquity or questionable due to an extraordinary com· plex of morphological features (the famous hoax of Piltdown). Largely as a consequence of this, there have grown up two main interpretations of man's phylogeny in the Pleistocene, one depending on the possible im­ portance of the suspicious fossils, and the other de­ pending only on the scanty, but well-dated, human fos­ sils enumerated above. The former interpretation recognizes an early, at least earlier, Middle Pleistocene separation of a morphologically modern (sapiens) lineage. The latter postulates progressive transforma· tion of primitive and variable Middle Pleistocene hu­ man populations into diverse Neanderthal and re­ lated, geographically distinctive, groups, as well as into incipiently sapiens peoples. The purpose of this paper is to discuss tI-1e significance of the :Middle Pleistocene human remains from Europe and North Africa for the resolution of this basic problem in the study of human phylogeny. COMPARATIVE STRATIGRAPHY AND ASSOCIATIONS THAMES RIVER: SWANSCOl\'IBE The Swanscombe human remains were recovered from gravel deposits exposed in the Barnfield pit, be­ tween Dartford and Gravesend, on the south bank of the lower Thames River valley. Marston (1937) dis­ covered a complete occipital in June, 1935, and a left parietal in March, 1936. Twenty years later Wymer re­ covered the right parietal of the same individual. The three fragments occurred in the same seam of sandy

Conducting osteological analyses on human skeletal material enhances our knowledge of these remains. Photos and descriptions are insufficient when communicating the wear, pathology, variation, or nuances of native populations. There are numerous laws, regulations, and financial dilemmas hindering our ability to further research human skeletal remains. Unfortunately, smaller institutions frequently find sophisticated imaging tools to be cost-prohibitive. Photographs lack sufficient detail when attempting to capture and convey an accurate representation of the unique structure, markings, and degenerationof human skeletal remains. Integrating new technology and techniques into this field has the potential to solve these dilemmas. CT scanning and file extracting creates virtual three-dimensional models and stores the information for future study. This removes the time limit surrounding this research (due to mandatory repatriation) and allows for thorough skeletal analyses. To assess this methodology, we conducted complete osteological analyses on two CT replicated human skeletal remains of two individuals uncovered during the archaeological excavation in Cabell County, West Virginia. In previous studies we analyzed the accuracy of the digital data by comparing physical human remains to the digitized versions. We hypothesized that the digital replicas allow a complete osteological analysis of the human skeletal material. Data were collected using human remains digitized on a GE VCT Medical CT scanner. The CT data were extracted into a 3D digital file for analysis while using the Standards for Data Collection from Human Skeletal Remains. Results show we can indicate identification of fundamental skeletal anatomy with all skeletal landmarks and basic osteometric measurements.

When human skeletal remains are discovered, there is initially little knowledge or explanation of their background. Executing a thorough osteological analysis of human skeletal remains can identify unknown information and provide new knowledge. Skeletal remains purchased from India until 1985 are believed to stem from a “legal” grave-robbing bone trade. A set of human remains housed at WVWC’s human anatomy lab, purchased in the early 1960s, display an “Origin India” sticker. Due to the origination, we decided to analyze the human remains. The purpose of this study is to conduct a complete osteological analysis on this set of human remains. Data were collected using Standards for Data Collection from Human Skeletal Remains and The Human Bone Manual. Specific bony features and abnormalities found during the analysis were compared to the given scientific norms to establish pathology. Preliminary anthropometric assessments show lumbosacral scoliosis. The remains appear to belong to a young adult (15-20) but age is difficult to infer due to post mortem alteration of the epiphyses. There is evidence of a potential calcium deficiency, which also affects epiphyseal fusion, which can alter age approximation. Sex appears ambiguous which supports the young age, but leaning male, based on the pubic symphysis and ventral arc. Bone remodeling around the sternum indicates fracture with complete healing. The tibiae show bowing; all articulations appear gracile, though some long bones show early osteoarthritis. Due to this controversial analysis, future research warrants a detailed computed tomography scan.

The recent advances in molecular biology have revolutionized all aspects of dentistry. DNA, the language of life yields information beyond our imagination, both in health or disease. DNA fingerprinting is a tool used to unravel all the mysteries associated with the oral cavity and its manifestations during diseased conditions. It is being increasingly used in analyzing various scenarios related to forensic science. The technical advances in molecular biology have propelled the analysis of the DNA into routine usage in crime laboratories for rapid and early diagnosis. DNA is an excellent means for identification of unidentified human remains. As dental pulp is surrounded by dentin and enamel, which forms dental armor, it offers the best source of DNA for reliable genetic type in forensic science. This paper summarizes the recent literature on use of this technique in identification of unidentified human remains.

The resolution of unidentified human remains (UHR) and long‐term missing persons (LTMP) cases is paramount for administrative, legal, and humanitarian reasons. There are various forensic profiling methods for human identification; however, their utility is dependent on several factors. First, UHR can be found in different stages of decomposition, so the availability and diversity of post‐mortem (PM) information will differ. Second, the availability and totality of the LTMP's ante‐mortem (AM) information will differ. Therefore, the suitability of existing methods will be dependent on the quality and quantity of PM and/or AM data available for comparison. Visual recognition is the simplest and quickest method, but typically not practiced or possible, owing to the altered, fragmented, or skeletonized state of UHR. Primary forensic profiling methods involve the comparison of fingerprint, dental, DNA, and medical data. Secondary forensic profiling methods from anthropology, radiology, geochemistry, and anatomy disciplines can provide supplementary evidence to support comparative identification approaches. Emerging forensic molecular technologies such as genomics, microbiomics, epigenetics, and proteomics, together with individual digital footprints from personal devices, offer new investigative leads for establishing identity. However, despite the success of these individual methods, their limitations must be considered when used in isolation. Through the development of a guiding forensic examination framework, this review endorses an interdisciplinary response to unidentified and missing persons investigations, where various forensic specialists collaboratively examine UHR using a suite of contemporary forensic profiling methods to produce multiple and/or different lines of evidence to link them effectively, efficiently, comprehensively, and systematically to LTMP.This article is categorized under:Forensic Science in Action/Crime Scene Investigation > Special Situations and InvestigationsForensic Biology > DNA Testing in Disaster Victim and Missing Persons IdentificationForensic Medicine > Medicolegal Death Investigation SystemsJurisprudence and Regulatory Oversight > Interdisciplinary Collaboration

Chapter 3 - Facilities design and workflow considerations for processing unidentified human skeletal remains

Human remains are present in a number of contexts. Some of these are archaeological burial sites, which can comprise individual or mass graves burials. Human remains are usually found buried (or cremated), but they can also be found in museums and in universities, as part of their anatomical collections. Human remains can be found in churches as relics, in ossuaries, and as part of objects. Hence human remains refer to not just a complete skeleton, but also apart of a bone or tooth, hair and mummified remains. In more recent forensic, police or medico-legal cases, human skeletal remains can be found in a number of contexts, such as fire scenes, natural disasters, clandestine graves, or on the surface in open areas (e.g. a woodland). One aspect ofphysical anthropology is that which studies human skeletal remains in order to reconstruct the past, understand human variation, and provide information about the deceased individuals, such as their age at death, sex, ancestry, stature, pathological conditions or traumatic injuries; the remains from medico-legal or police cases fall under the branch offorensic anthropology.

A new set of multiplexed PCR primers has been applied to the analysis of human skeletal remains to determine their efficacy in analyzing degraded DNA. These primer sets, known as Miniplexes, produce shorter amplicons (50-280 base pairs (bp)) than standard short tandem repeat (STR) kits, but still utilize the 13 CODIS STR loci, providing results that are searchable on national DNA databases. In this study, a set of 31 different human remains were exposed to a variety of environmental conditions, extracted, and amplified with commercial and Miniplex DNA typing kits. The amplification efficiency of the Miniplex sets was then compared with the Promega PowerPlex 16 system. Sixty-four percent of the samples generated full profiles when amplified with the Miniplexes, while only 16% of the samples generated full profiles with the Powerplex 16 kit. Complete profiles were obtained for 11 of the 12 Miniplex loci with amplicon sizes less than 200 bp. These data suggest smaller PCR amplicons may provide a useful alternative to mitochondrial DNA for anthropological and forensic analysis of degraded DNA from human skeletal remains.

We present a proposed standard regarding the use of contemporary human skeletal remains in education, training, and research contexts in forensic anthropology. This document was generated by the Anthropology subcommittee of the Organization of Scientific Area Committees for Forensic Science and is currently under review for publication by the American Academy of Forensic Sciences', Academy Standards Board as a joint venture. The OSAC is federally funded and charged with drafting standards documents for various aspects of forensic science in the United States, while the ASB is an accredited Standards Development Organization which reviews and publishes proposed standards documents. The use of real human skeletal remains is crucial for students to learn how to identify human remains and develop various competencies as part of their education and training on their path to becoming professionals; they are also required for research. However, many recent transgressions have highlighted the need for the standardization of practices for the ethical treatment of human remains. We argue that the foundations of the ethical treatment of human remains are: informed consent, deathcare, and service to communities. This document provides a framework for informed consent based on the remains' origin (donated, unclaimed, or unidentified) and how obtained permissions dictate the ethical use of human remains from different contexts. Guidance is also provided for the use of information associated with remains (e.g., images, measurements), data associated with the remains, and their final disposition.

Human migration patterns are of interest to scientists representing many fields. Theories have been posited to explain modern human evolutionary expansion, the diversity of human culture, and the motivational factors underlying an individual or group decision to migrate. Although the research question and subsequent approach may vary between disciplines, one thread is ubiquitous throughout most migration studies: why do humans migrate and what is the result of such an event? While the determination of individual attributes such as age, sex, and ancestry is often integral to migration studies, the positive identification of human remains is usually irrelevant. However, the positive identification of a deceased is paramount to a forensic investigation in which human remains have been recovered and must be identified. What role, if any, might the study of human movement patterns play in the interpretation of evidence associated with unidentified human remains? Due to increasing global mobility in the world's populations, it is not inconceivable that an individual might die far away from his or her home. If positive identification cannot immediately be made, investigators may consider various theories as to how or why a deceased ended up in a particular geographic location. While scientific evidence influences the direction of forensic investigations, qualitative evaluation can be an important component of evidence interpretation. This review explores several modern human migration theories and the methodologies utilized to identify evidence of human migratory movement before addressing the practical application of migration theory to forensic cases requiring the identification of human remains.

Forensic anthropology deals with the identification of human remains in a legal setting. The primary role of a forensic anthropologist is to evaluate human skeletal remains and ascertain the biological profile of the deceased. Forensic anthropologists thus use their expertise to assist medical examiners, as well as law enforcement agencies. Forensic anthropology was not recognised as a subject specialty of much significance in most parts of the world for a long time. However, now its status has changed, mostly in the USA and parts of Europe. The scope of the subject ranges from evaluation of human skeletal remains to the identification of the living. In the new millennium, the role of forensic anthropologists includes disaster victim identification, photographic superimposition, facial image reconstruction in CCTV footage, gait pattern analysis from video surveillance, ascertaining the age of an individual in absence of legal documents and paedo-pornographic materials. Despite its relevance in human identification, the development of forensic anthropology in India is not very encouraging. Only a few institutions in India include forensic anthropology as a part of the forensic science degree programme, and there are no certified courses in anthropology either. Likewise, there are limited employment opportunities for forensic anthropologists in medical institutes/organisations/universities. This comprehensive review focuses on the status and development of forensic anthropology in India and emphasises the need for its recognition as a specialty of significance. The article also throws light on the comparison of its status in the Indian context vis a vis developed nations.