Abstract
SHBG was highly purified by a 4-step method. Molecular weight, isoelectric point and DHT-binding were determined. Antibodies were generated and the well known transferrin crossreactivity was eliminated by affinity chromatography. SHBG as standard was not suitable for the RIA because of insufficient stability and was replaced by pregnancy serum. The tracer had to be chromatographed before each use. SHBG levels measured with this RIA are in the same range as has been reported in literature.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
