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Abstract
Cryopreservation-thawing of human semen was found to reduce the level of antioxidant activity surrounding the sperm, which may negatively affect post-cryopreservation (post-thaw) recovery of sperm motility. Therefore, the current manufactured cryoprotectant media have been supplemented with certain antioxidants to preserve the loss in seminal antioxidant activity. In this study, we aimed to explore the correlation between total antioxidant capacity (TAC) of human semen samples before cryopreservation and the post-thaw recovery of sperm motility. Normal semen specimens (n = 77) were recruited in this study. Sperm motility was measured for each semen sample before and after cryopreservation and the post-thaw recovery of sperm motility was calculated. Seminal TAC was measured spectrophotometrically before cryopreservation for each semen sample using the sensitive cupric ion-reducing antioxidant capacity (CUPRAC) method. The results from this study showed that the post-thaw recovery of sperm motility is negatively correlated (p = 0.0404, p = 0.0402) with the absorbance at 450 nm and the values of seminal TAC in terms of µM Trolox equivalents, as evaluated by CUPRAC, respectively. In conclusion, the total antioxidant reservoir in each ejaculated semen specimen could be a factor in determining the post-thaw recovery of sperm motility toward lower recovery for semen specimens of high antioxidant content.
Highlights
Semen cryopreservation is a procedure used in a variety of conditions that may affect semen quality such as surgical infertility intervention, chemotherapy treatment, seronegativity confirmation of viral infection (e.g., human immunodeficiency virus (HIV), hepatitis), and assisted reproductive technologies [1]
We aimed to investigate the correlation between total antioxidant capacity (TAC) of semen samples before cryopreservation and the post-thaw recovery of sperm motility
In accordance with our hypothesis, the results from this work revealed that human semen samples
Summary
Semen cryopreservation is a procedure used in a variety of conditions that may affect semen quality such as surgical infertility intervention, chemotherapy treatment, seronegativity confirmation of viral infection (e.g., human immunodeficiency virus (HIV), hepatitis), and assisted reproductive technologies [1]. Various studies have targeted the improvement of this procedure by different means to ensure a better sperm recovery One example of such a specific intention in research and technology was to augment the antioxidant system surrounding the sperm before cryopreservation, which may increase the post-cryopreservation (post-thaw) sperm recovery, and, the ability of cryopreserved semen to achieve fertilization. This is, most of time, performed by enhancing/enriching the cryoprotectant medium of semen with some potential antioxidants [2,3,4]
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