Abstract
BackgroundCurrently, clinical examination, ultrasound scanning (with or without fine needle aspiration cytology), preoperative CT-scan and MRI are available for the differential diagnosis of parotid gland swelling. A preliminary non-invasive salivary diagnostic tool may be helpful in the clinical decision making process. Altered salivary micro-RNA (miRNA) expression levels have been observed in saliva from patients with various cancers. Therefore, we investigated miRNA expression levels in saliva samples from patients with a parotid gland neoplasm using Human miRNA cards in comparison to controls.ResultsIn the discovery phase, eight miRNAs were identified having different expression levels in patients compared to controls. In the validation phase, the differences in miRNA expression levels between patients and controls were confirmed for seven out of eight discovered miRNAs (p < 0.001). A combination of two miRNAs yielded a receiver-operator-characteristics curve with an AUC of 0.94 (95% CI: 0.87–1.00; sensitivity 91%; specificity 86%). Validation of discovered miRNAs in segregated collected parotid saliva revealed that expression of these miRNAs differ between whole saliva and parotid saliva.ConclusionsA two miRNA combination can predict the presence of a parotid gland neoplasm. Furthermore, this study suggested that the identified, patient-specific, salivary miRNAs were not derived from the parotid gland itself.
Highlights
MicroRNAs are small non-coding RNAs consisting of 19–25 nucleotides
A combination of two miRNAs yielded a receiver-operator-characteristics curve with an Areas under the curve (AUC) of 0.94
A two miRNA combination can predict the presence of a parotid gland neoplasm
Summary
MicroRNAs (miRNAs) are small non-coding RNAs consisting of 19–25 nucleotides They are involved in various cellular processes such as cell differentiation, proliferation and survival. Specific miRNA expression profiles were identified in a number of cancers [1,5,6,7,8,9]. Fluid-specific miRNA expression profiles have been identified [1,10,11]. Differences in miRNA expression profiles were found in cystic fluid of intraductal papillary mucinous neoplasm of the pancreas and in urine samples from patients with urinary bladder cancer. In saliva samples from patients with esophageal cancer, the expression level of three miRNAs (hsa-miR-10bÃ, hsa-miR-144, and hsa-miR-451) was significantly higher compared to the control group. Altered salivary micro-RNA (miRNA) expression levels have been observed in saliva from patients with various cancers. We investigated miRNA expression levels in saliva samples from patients with a parotid gland neoplasm using Human miRNA cards in comparison to controls
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